Soil Biology; Laboratory Manual

Soil Biology; Laboratory Manual

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This historic book may have numerous typos and missing text. Purchasers can usually download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1917 edition. Excerpt: ...time between each to avoid cracking. Place 1 loopful of water on the cover slip. Spread by pulling and do it gently. Care at this point is highly essential. The end of the platinum wire may be used for this purpose. First fix with gentle heat and then apply the mordant (Loeffler's Flagella Stain) which should be filtered on to the cover slip until completely flooded and heat until steam arises (use care not to overheat). Hold the cover slip with cornet forceps and keep the finger extended over it when passing through the flame. Heat-minute. Wash in water and blot with filter paper. Stain with the carbolfuchsin, taking care to completely flood the cover slip so that no material will be precipitated out during the 3 minutes heating. Wash and examine. Mount in balsam if a satisfactory stain is obtained. Keep accurate notes on the procedure. ADVANCED PRACTICE 5. CROSS INOCULATION OF LEGUMES. Some valuable cross inoculations have already been made by this station. There are no doubt many other similar cross inoculations which may be made. The following method will serve to enable advanced students to test some of these possibilities. Sterilize clean white sand in 600 cc. beakers. Add 5 cc. sterile plant food solution (nitrogen omitted) in 140 cc. of sterile water. Sterilize the legume seeds in 1-500 mercuric chloride solution and with a sterile pipette add the legume bacteria in the following experiment: 1-5, 1st legume, inoculated with bacteria common to it. 6-10, 2nd legume, inoculated with bacteria of legume No. 1. 11-15, 2nd legume, inoculated with bacteria common to it. 16-20, 1st legume, inoculated with bacteria of legume No. 2. 21-25, 1st legume, uninoculated (sterile). 26-30, 2nd legume, uninoculated (sterile). Failure to prove out...This historic book may have numerous typos and missing text. Purchasers can usually download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1917 edition.


Title:Soil Biology; Laboratory Manual
Author: Albert Lemuel Whiting
Publisher:Theclassics.Us - 2013-09
ISBN-13:

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